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TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template
(Plasmid #167971)


Item Catalog # Description Quantity Price (USD)
Plasmid 167971 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Mutation
    homology arms contain point mutations to disrupt crRNA binding sites used and point mutations specific to WTC genome
  • Entrez Gene
    TP63 (a.k.a. AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L)
  • Tag / Fusion Protein
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAGGAAACAGCTATGAC
  • 3′ sequencing primer TGTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template was a gift from Emma Rawlins (Addgene plasmid # 167971 ; ; RRID:Addgene_167971)
  • For your References section:

    A functional genetic toolbox for human tissue-derived organoids. Sun D, Evans L, Lim K, Rawlins EL. bioRxiv February 24, 2021 10.1101/2020.05.04.076067