pCS2-zfIl34
(Plasmid #168112)

• Purpose: Express zebrafish Il34, mRNA synthesis
• Depositing Lab: Petr Bartunek
• Publication: Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi: 10.1182/bloodadvances.2021005459.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Total vector size (bp): 4752
• Vector type: Mammalian Expression ; Vertebrate expression (zebrafish, chicken, xenopus)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Il34
• Alt name: IL-34, interleukin 34
• Species: D. rerio (zebrafish)
• Insert Size (bp): 657
• GenBank ID: NM_001128701
• Entrez Gene: il34 (a.k.a. IL-34, si:ch211-239j19.3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: ATTTAGGTGACACTATAG

Resource Information

• Supplemental Documents:
  • pCS2-zfIl34.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry