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Go1-CASE
(Plasmid #168123)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 168123 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Clontech-style N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GNAO1, GNB3, GNG9
  • Species
    H. sapiens (human)
  • Mutation
    NLuc is inserted at L91/G92 within GNAO1
  • Entrez Gene
    GNAO1 (a.k.a. DEE17, EIEE17, G-ALPHA-o, GNAO, HG1G, HLA-DQB1, NEDIM)
  • Entrez Gene
    GNB3 (a.k.a. CSNB1H)
  • Entrez Gene
    GNGT2 (a.k.a. G-GAMMA-8, G-GAMMA-C, GNG9, GNGT8)
  • Tag / Fusion Protein
    • cpVenus on GNG9 (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Go1-CASE was a gift from Gunnar Schulte (Addgene plasmid # 168123 ; http://n2t.net/addgene:168123 ; RRID:Addgene_168123)
  • For your References section:

    Quantitative assessment of constitutive G protein-coupled receptor activity with BRET-based G protein biosensors. Schihada H, Shekhani R, Schulte G. Sci Signal. 2021 Sep 7;14(699):eabf1653. doi: 10.1126/scisignal.abf1653. Epub 2021 Sep 7. 10.1126/scisignal.abf1653 PubMed 34516756