XE73 TM Beta-catenin 1-myc in CS2+
(Plasmid #16834)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)
• Sequence Information:

  • Depositor Sequences: None.
  • Addgene Sequences: Partial (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	16834	Plasmid sent as bacteria in agar stab	1	$65	Add to Cart

Available to Academic and Nonprofits Only

Backbone

• Vector backbone: pCS2+
  (Search Vector Database)
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TM beta-catenin 1-myc
• Alt name: beta catenin
• Species: X. laevis (frog)
• Entrez Gene: ctnnb1-b (a.k.a. beta-catenin, ctnnb, ctnnb1)
• Tag / Fusion Protein:
  • c-myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Terms and Licenses:
  • UBMTA

Depositor Comments

TM of N-cadherin to NH2 terminal deletion of Beta-catenin myc.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.