XE73 TM Beta-catenin 1-myc in CS2+
(Plasmid
#16834)
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Depositing Lab
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Publication
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Sequence Information
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Depositor Sequences: None.
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Addgene Sequences: Partial (1)
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 16834 | Plasmid sent as bacteria in agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTM beta-catenin 1-myc
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Alt namebeta catenin
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SpeciesX. laevis (frog)
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Entrez Genectnnb1-b (a.k.a. beta-catenin, ctnnb, ctnnb1)
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Tag
/ Fusion Protein
- c-myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Terms and Licenses
Depositor Comments
TM of N-cadherin to NH2 terminal deletion of Beta-catenin myc.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE73 TM Beta-catenin 1-myc in CS2+ was a gift from Randall Moon (Addgene plasmid # 16834)
Map uploaded by the depositor.
