Addgene - XE73 TM Beta-catenin 1-myc in CS2+ Plasmid Data

Plasmid 16834: XE73 TM Beta-catenin 1-myc in CS2+

Gene/insert name:
TM beta-catenin 1-myc
Alt name:
beta catenin
Species:
X. laevis (frog)
Entrez Gene:
ctnnb1-b (beta-catenin, ctnnb, ctnnb1)
Fusion protein or tag:
c-myc
Terminal:
C terminal on insert
Vector backbone:
pCS2+
(Search Vector Database)
Vector type:
Xenopus Expression
Backbone size w/o insert (bp):
4095
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Randall Moon
Terms and Licenses:
UBMTA

Comments:

TM of N-cadherin to NH2 terminal deletion of Beta-catenin myc.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16834" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only

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This is commonly requested with

16842: XE28 XBC 40