XE73 TM Beta-catenin 1-myc in CS2+
(Plasmid #16834)

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Depositing Lab

Randall Moon
Publication

Xenopus Expression Plasmids (unpublished)
Price

$65 (USD)

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Available to Academic and Nonprofits Only

Backbone

Vector backbone
pCS2+
(Search Vector Database)
Backbone size w/o insert (bp) 4095
Vector type
Xenopus Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Sequence Information

Depositor Sequences

None.
Addgene Sequences

Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

Gene/Insert name
TM beta-catenin 1-myc
Alt name
beta catenin
Species
X. laevis (frog)
Entrez Gene
ctnnb1-b (a.k.a. beta-catenin, ctnnb, ctnnb1)
Tag / Fusion Protein
- c-myc (C terminal on insert)

Cloning Information

Cloning method Restriction Enzyme
5′ sequencing primer SP6
3′ sequencing primer T7

(Common Sequencing Primers)

Resource Information

Terms and Licenses
- UBMTA

Depositor Comments

TM of N-cadherin to NH2 terminal deletion of Beta-catenin myc.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

XE73 TM Beta-catenin 1-myc in CS2+ was a gift from Randall Moon (Addgene plasmid # 16834)

Map uploaded by the depositor.

XE73 TM Beta-catenin 1-myc in CS2+ (Plasmid #16834)