Addgene - XE73 TM Beta-catenin 1-myc in CS2+ Plasmid Data

Plasmid 16834: XE73 TM Beta-catenin 1-myc in CS2+

Gene/insert name:
TM beta-catenin 1-myc
Alt name:
beta catenin
Species:
X. laevis (frog)
Entrez Gene:
ctnnb1-b (beta-catenin, ctnnb, ctnnb1)
Fusion protein or tag:
c-myc
Terminal:
C terminal on insert
Vector backbone:
pCS2+
(Search Vector Database)
Vector type:
Xenopus Expression
Backbone size w/o insert (bp):
4095
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Randall Moon
Terms and Licenses:
UBMTA

Comments:

TM of N-cadherin to NH2 terminal deletion of Beta-catenin myc.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16834" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only

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This is commonly requested with

16842: XE28 XBC 40