pGEX-4T-2/Rabaptin-5:R5BD
(Plasmid #168808)

• Purpose: Production of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members
• Depositing Lab: Guangpu Li
• Publication: Qi et al Methods Mol Biol. 2015;1298:259-70. doi: 10.1007/978-1-4939-2569-8_22.

Backbone

• Vector backbone: pGEX-4T-2
• Backbone manufacturer: Pharmacia/GE Healthcare
• Backbone size w/o insert (bp): 4970
• Total vector size (bp): 5335
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rab5-binding domain (aa739-862) of Rabaptin-5
• Species: H. sapiens (human)
• Insert Size (bp): 374
• GenBank ID: X91141.1
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CACGTTTGGTGGTGG
• 3′ sequencing primer: ccgaaacgcgcgagg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry