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(Plasmid #168808)


Item Catalog # Description Quantity Price (USD)
Plasmid 168808 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Pharmacia/GE Healthcare
  • Backbone size w/o insert (bp) 4970
  • Total vector size (bp) 5335
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Rab5-binding domain (aa739-862) of Rabaptin-5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CACGTTTGGTGGTGG
  • 3′ sequencing primer ccgaaacgcgcgagg
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-2/Rabaptin-5:R5BD was a gift from Guangpu Li (Addgene plasmid # 168808 ; ; RRID:Addgene_168808)
  • For your References section:

    Determination of Rab5 activity in the cell by effector pull-down assay. Qi Y, Liang Z, Wang Z, Lu G, Li G. Methods Mol Biol. 2015;1298:259-70. doi: 10.1007/978-1-4939-2569-8_22. 10.1007/978-1-4939-2569-8_22 PubMed 25800849