|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16931||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)780
Entrez Genesnai1a (a.k.a. MGC109820, cb643, sna-1, sna1, snai1, snail1, zgc:109820)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
Antisense probe: cut with EcoR1 and transcribe with T3.
Sense probe: cut with BamH1 and transcribe with T7.
Plasmid History: Constructed by Greg Kelly, 1/94.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZP10 Snail was a gift from Randall Moon (Addgene plasmid # 16931 ; http://n2t.net/addgene:16931 ; RRID:Addgene_16931)