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Plasmid 16984: XP6 Banded hh
  • banded hedgehog

  • banded hh

  • 2500

  • X. laevis (frog)

  • ihh (bhh, X-hh, bhh-a)

  • pBluescript KS
    (Search Vector Database)

  • Stratagene

  • Bacterial Expression

  • 3000

  • EcoRI

  • No

  • EcoRI

  • No

  • T7 List of Sequencing Primers

  • T3

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • Randall Moon

  • UBMTA

Comments: 

Xenopus Hedgehog. Antisense are generated as listed:

Xenopus banded hedgehog: Linearize clone XIL in Bluescript KS with BamH1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.

Xenopus sonic hedgehog: Linearize clone X32 in Bluescript KS with Bam H1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.

Xenopus cephalic hedgehog:Linearize clone X30 in pT7TS with Sal 1 and transcribe with T7.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16984" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with