1
Plasmid 16984: XP6 Banded hh
  • banded hedgehog

  • banded hh

  • 2500

  • X. laevis (frog)

  • ihh (bhh, X-hh, bhh-a)

  • pBluescript KS
    (Search Vector Database)

  • Stratagene

  • Bacterial Expression

  • 3000

  • EcoRI

  • No

  • EcoRI

  • No

  • T7 List of Sequencing Primers

  • T3

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • Randall Moon

  • UBMTA

Comments: 

Xenopus Hedgehog. Antisense are generated as listed:

Xenopus banded hedgehog: Linearize clone XIL in Bluescript KS with BamH1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.

Xenopus sonic hedgehog: Linearize clone X32 in Bluescript KS with Bam H1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.

Xenopus cephalic hedgehog:Linearize clone X30 in pT7TS with Sal 1 and transcribe with T7.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16984" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with