pBS-ACTB-C200-GFPfr2
(Plasmid #170726)

• Purpose: HR donor vector for ACTB gene with the revised marker sequence for ASHRA.
• Depositing Lab: Natsuko Chiba
• Publication: HR donor vector for ACTB gene with the revised marker sequence (unpublished)

Backbone

• Vector backbone: pBlueScript II SK (+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2898
• Total vector size (bp): 3295
• Vector type: HR donor vector for human ACTB gene.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Donor sequence to ACTB gene for HR with the revised marker sequence
• Species: H. sapiens (human)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: taatacgactcactatagg
• 3′ sequencing primer: aattaaccctcactaaagg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Using this plasmid as a substitute for pBS-ACTB-C200-GFPfr1, less non-specific amplification is observed in the downstream qPCR.
The following primer set can be used to detect the HR product.
GFPrev2-F: 5'-CTTCAAGATCCGCCACAACGT-3'
ACTB-common-R1: 5'-GTGCAATCAAAGTCCTCGGC-3'