pPPC009.106
(Plasmid
#171142)
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PurposeExpression of J106 scRNA on pBBR1-KmR plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171142 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBBR1-MCS2
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Backbone manufacturerKenneth M. Peterson
- Backbone size w/o insert (bp) 5148
- Total vector size (bp) 5570
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsMedium Copy in P. putida
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameJ106 scRNA
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gRNA/shRNA sequenceAGGACGCCTTTGGTAACCGC
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SpeciesSynthetic
- Promoter BBa_J23119
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer tttcccagtcacgacgttgt
- 3′ sequencing primer tgaccatgattacgccaagc (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Alternative names: pCK033.06, pBBR1-KmR_J106
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPPC009.106 was a gift from Jesse Zalatan (Addgene plasmid # 171142 ; http://n2t.net/addgene:171142 ; RRID:Addgene_171142) -
For your References section:
Portable bacterial CRISPR transcriptional activation enables metabolic engineering in Pseudomonas putida. Kiattisewee C, Dong C, Fontana J, Sugianto W, Peralta-Yahya P, Carothers JM, Zalatan JG. Metab Eng. 2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 10.1016/j.ymben.2021.04.002. 10.1016/j.ymben.2021.04.002 PubMed 33930546
