E2_pENTR_R4R3_T CTP
(Plasmid
#171736)
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PurposeptpTALECD vector assembly, Entry clone 2 with attR4 and R3
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171736 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePINII
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Vector typeMultisite Gateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameA terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Multisite Gateway entry vector to terminate E1 ORF and having RPS5A promoter with RecA1 plastid targeting signal for E3 ORF.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
E2_pENTR_R4R3_T CTP was a gift from Shin-ichi Arimura (Addgene plasmid # 171736 ; http://n2t.net/addgene:171736 ; RRID:Addgene_171736) -
For your References section:
Targeted base editing in the plastid genome of Arabidopsis thaliana. Nakazato I, Okuno M, Yamamoto H, Tamura Y, Itoh T, Shikanai T, Takanashi H, Tsutsumi N, Arimura SI. Nat Plants. 2021 Jul;7(7):906-913. doi: 10.1038/s41477-021-00954-6. Epub 2021 Jul 1. 10.1038/s41477-021-00954-6 PubMed 34211131
