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M27 NacMS>luc
(Plasmid #17177)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17177 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nacre promoter
  • Alt name
    nacre
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    800
  • Mutation
    All three Tcf/Lef sites are mutated
  • GenBank ID
    AF211890
  • Entrez Gene
    mitfa (a.k.a. nacre, z3A.1)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Description: zebrafish nacre promoter with all three LEF sites mutated, driving luciferase reporter. This is a control construct.

Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M27 NacMS>luc was a gift from Randall Moon (Addgene plasmid # 17177 ; http://n2t.net/addgene:17177 ; RRID:Addgene_17177)