M27 NacMS>luc
(Plasmid
#17177)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 17177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGL3
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Backbone manufacturerpromega
- Backbone size w/o insert (bp) 4818
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenacre promoter
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Alt namenacre
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)800
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MutationAll three Tcf/Lef sites are mutated
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GenBank IDAF211890
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Entrez Genemitfa (a.k.a. nacre, z3A.1)
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
Depositor Comments
Description: zebrafish nacre promoter with all three LEF sites mutated, driving luciferase reporter. This is a control construct.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M27 NacMS>luc was a gift from Randall Moon (Addgene plasmid # 17177 ; http://n2t.net/addgene:17177 ; RRID:Addgene_17177)
Map uploaded by the depositor.
