M27 NacMS>luc
(Plasmid #17177)

• Depositing Lab: Randall Moon
• Publication: Other Plasmids (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	17177	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pGL3
  (Search Vector Database)
• Backbone manufacturer: promega
• Backbone size w/o insert (bp): 4818
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: nacre promoter
• Alt name: nacre
• Species: D. rerio (zebrafish)
• Insert Size (bp): 800
• Mutation: All three Tcf/Lef sites are mutated
• GenBank ID: AF211890
• Entrez Gene: mitfa (a.k.a. nacre, z3A.1)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: LucNRev

Resource Information

• Terms and Licenses:
  • UBMTA
  • Luciferase Limited Use Label License

Depositor Comments

Description: zebrafish nacre promoter with all three LEF sites mutated, driving luciferase reporter. This is a control construct.

Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.