Addgene - M36 Dpr C-terminal GST Plasmid Data

Plasmid 17192: M36 Dpr C-terminal GST

Gene/insert name:
XDpr
Alt name:
Dapper
Alt name:
dact1
Insert size:
1200
Species:
X. laevis (frog)
Entrez Gene:
dact1-b (dpr, dpr1, dact1, frodo, hdpr1, Dapper, thyex3, dapper1)
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Vector backbone:
pGEX
(Search Vector Database)
Vector type:
Bacterial Expression
Cloning site 5':
EcoRI
Site destroyed during cloning:
Unknown
Cloning site 3':
XbaI
Site destroyed during cloning:
Unknown
5' sequencing primer:
pGEX 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Randall Moon
Terms and Licenses:
MTA

Comments:

Description: Coding sequence of original XDpr prey clone (M35) cloned into pGEX vector as a 1.2 kb EcoR1-Xba1 fragment (Xba site is DAM methylated).

Reference: Cheyette, B. et al., 2002, Developmental Cell 2: 449-461.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17192" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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