pACYCDuet_DL121_WTTS
(Plasmid #171954)

• Purpose: Selection vector for chimeric fusion of ecDHFR and asLOV2.
• Depositing Lab: Kimberly Reynolds
• Publication: McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi: 10.7554/eLife.68346.

Backbone

• Vector backbone: pACYCDuet-1
• Backbone size w/o insert (bp): 3907
• Total vector size (bp): 5633
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Fusion of ecDHFR and asLOV2 at position 121 of DHFR
• Alt name: DL121
• Species: Escherichia coli
• Insert Size (bp): 920
• Promoter: T7
• Tag / Fusion Protein:
  • 8-His tag (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Gene/Insert 2

• Gene/Insert name: Thymidylate Synthase
• Alt name: TYMS
• Alt name: Thymidylate Synthetase
• Species: E. coli
• Insert Size (bp): 806
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Supplemental Documents:
  • pACYCDuet_DL121_WTTS.gb
• A portion of this plasmid was derived from a plasmid made by: Kimberly Reynolds

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Introduced in Reynolds et al. Cell 2011, PMID: 22196731.