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M71 pGL3 Sp5l P1203 mut
(Plasmid #17209)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17209 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sp5l promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1250
  • Mutation
    6 of the putative Tcf/Lef sites mutated
  • Entrez Gene
    sp5l (a.k.a. Spr2, YF-335, cb577, fc23b01, sb:cb577, wu:fc23b01)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon.
(as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 clone.
upstream of firefly luciferase in the pGL3 Basic promoter. 6 of the putative Tcf/Lef sites mutated.
(all introduce additional restriction sites); cloned by PCR-based in vitro synthesis from 28 overlapping.
oligos. cloned NdeI / HindIII to exchange wild-type fragment in pGL3 Sp5l P1203.

Species of Sequence Origin: zebrafish.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M71 pGL3 Sp5l P1203 mut was a gift from Randall Moon (Addgene plasmid # 17209 ; http://n2t.net/addgene:17209 ; RRID:Addgene_17209)