M71 pGL3 Sp5l P1203 mut
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17209||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Growth in Bacteria
Gene/Insert nameSp5l promoter
SpeciesD. rerio (zebrafish)
Insert Size (bp)1250
Mutation6 of the putative Tcf/Lef sites mutated
Entrez Genesp5l (a.k.a. Spr2, YF-335, cb577, fc23b01, sb:cb577, wu:fc23b01)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon.
(as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 clone.
upstream of firefly luciferase in the pGL3 Basic promoter. 6 of the putative Tcf/Lef sites mutated.
(all introduce additional restriction sites); cloned by PCR-based in vitro synthesis from 28 overlapping.
oligos. cloned NdeI / HindIII to exchange wild-type fragment in pGL3 Sp5l P1203.
Species of Sequence Origin: zebrafish.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M71 pGL3 Sp5l P1203 mut was a gift from Randall Moon (Addgene plasmid # 17209 ; http://n2t.net/addgene:17209 ; RRID:Addgene_17209)