NmMetNI-WT
(Plasmid #172114)

• Purpose: Expression of MetN and MetI membrane proteins
• Depositing Lab: Douglas Rees
• Publication: Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi: 10.7554/eLife.69742.

Backbone

• Vector backbone: pET21b+
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5245
• Total vector size (bp): 6916
• Modifications to backbone: Added 4 more His to make a total of 10X His. Added Enterokinase after His tag. Removed original pelB sequence from vector backbone.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: MetN
• Species: N.Neisseria meningitidis serogroup B (strain MC58)
• Insert Size (bp): 738
• Promoter: T7 Promoter
• Tags / Fusion Proteins:
  • 10x His Tag (N terminal on insert)
  • Enterokinase (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: MetN Custom Gene Specific Primer
• 3′ sequencing primer: MetN Custom Gene Specific Primer

Gene/Insert 2

• Gene/Insert name: MetI
• Species: N.Neisseria meningitidis serogroup B (strain MC58)
• Insert Size (bp): 687
• Promoter: T7 Promoter

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: MetI Custom Gene Specific Primer
• 3′ sequencing primer: MetI Custom Gene Specific Primer

Resource Information

• Supplemental Documents:
  • nmlm-july-2021.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry