pEx-insertion-erm
(Plasmid
#172210)
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Purpose(Empty Backbone) For genetic insertion in erythromycin-sensitive Prevotella copri strains
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172210 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepExchange-tdk
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Backbone manufacturerKoropatkin, N.M. (PMID: 18611383)
- Backbone size (bp) 4172
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Vector typeGenetic insertion in Prevotella copri
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Selectable markersErythromycin in Prevotella copri
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Growth instructionsSupplementing Media with 0.3 mM 2,6-Diaminopimelic acid (DAP) is required if grown in B2155 E.coli strain
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEx-insertion-erm was a gift from Till Strowig (Addgene plasmid # 172210 ; http://n2t.net/addgene:172210 ; RRID:Addgene_172210) -
For your References section:
A versatile genetic toolbox for Prevotella copri enables studying polysaccharide utilization systems. Li J, Galvez EJC, Amend L, Almasi E, Iljazovic A, Lesker TR, Bielecka AA, Schorr EM, Strowig T. EMBO J. 2021 Dec 1;40(23):e108287. doi: 10.15252/embj.2021108287. Epub 2021 Oct 21. 10.15252/embj.2021108287 PubMed 34676563
