pTD56
(Plasmid #172895)

• Purpose: mKate2^SEC^AID* vector with ccdB markers for cloning homology arms
• Depositing Lab: David Reiner
• Publication: Reiner lab plasmids for self-excising CRISPR knock-in (unpublished)

Backbone

• Vector backbone: pDD285
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mKate2
• Species: Entacmaea quadricolor

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pTD56--mKate2^SEC^Degron ccdB.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mKate2^SEC^Degron