pRS41K-mCherry-sfGFP (pJJF001)
(Plasmid #173457)

• Purpose: C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer
• Depositing Lab: Anton Khmelinskii
• Publication: Fung et al Methods Mol Biol. 2022;2378:85-100. doi: 10.1007/978-1-0716-1732-8_6.

Backbone

• Vector backbone: pRS41K-CEN ARS kanMX (type I donor template)
• Backbone size w/o insert (bp): 5230
• Total vector size (bp): 6685
• Modifications to backbone: Vector pRS41K-CEN ARS kanMX (type I donor template) (pMaM482) was digested with BamHI and SpeI. Insert mCherry-sfGFP was PCR amplified from pFA6a-mCherry-sfGFP-kanMX (pMaM17). Vector and insert were assembled by Gibson and transformed into DH5α E. coli.
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mCherry-sfGFP
• Insert Size (bp): 1461
• Promoter: no promoter

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ttaccctgttatccctac
• 3′ sequencing primer: atcgatgaattcgagctcg

Resource Information

• Supplemental Documents:
  • pRS41K-mCherry-sfGFP (pJJF001).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry