Addgene - pFLAG-4EHP(K134R) Plasmid Data

Plasmid 17348: pFLAG-4EHP(K134R)

Gene/insert name:
4EHP
Insert size:
750
Species:
H. sapiens (human)
GenBank ID:
NM_004846
Entrez Gene:
EIF4E2 (4EHP, IF4e, 4E-LP, EIF4EL3)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Mutation:
K134R
Vector backbone:
pFlag-CMV2
(Search Vector Database)
Backbone manufacturer:
Sigma
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Dong-Er Zhang
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: ISG15 modification of the eIF4E cognate 4EHP enhances cap structure-binding activity of 4EHP. Okumura et al (Genes Dev. 2007 Feb 1. 21(3):255-60. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17348" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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