pDSARN-vasa-eSpCas9sv40
(Plasmid #173670)

• Purpose: eSpCas9 expression vector for Anopheles
• Depositing Lab: Eric Marois
• Publication: Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881. doi: 10.1371/journal.ppat.1010881. eCollection 2022 Oct.

Backbone

• Vector backbone: pDSARN
• Backbone manufacturer: Marois lab
• Backbone size w/o insert (bp): 4336
• Total vector size (bp): 11141
• Vector type: Insect Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eSpCas9 with Anopheles vasa promoter and SV40 terminator
• Species: Synthetic
• Insert Size (bp): 6805
• Mutation: mutations in Cas9 reducing off-target activity (Slaymaker et al., 2016)
• Promoter: vasa

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: M13F

Resource Information

• Supplemental Documents:
  • pDSARN-agVasa-eSpCas9_756.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Transgenesis vector to create eSpCas9-expressing mosquitoes. eSpCas9 mutant from Zhang lab (Slaymaker et al., 2016) producing fewer off-target mutations. 3xP3-DsRed selection marker. Can also be used as helper plasmid in embryo microinjection.