pHelper_O
(Plasmid #173783)

• Purpose: The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.
• Depositing Lab: Keith Shearwin
• Publication: Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.1021/acssynbio.1c00215.

Backbone

• Vector backbone: pHelper
• Backbone manufacturer: A portion of this plasmid was derived from a plasmid made by Haldimann and Wanner J. Bact 183, 6384-6393 (2001).
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: 186 Integrase
• Species: Other
• Mutation: E59G - please see depositor comments

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pHelper_O.APE

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor confirms E59G in 186 Integrase does not affect plasmid function.