pE_Cre_lambdaCos
(Plasmid #173785)

• Purpose: The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.
• Depositing Lab: Keith Shearwin
• Publication: Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.1021/acssynbio.1c00215.

Backbone

• Vector backbone: pE-FLP
• Vector type: Cre/Lox, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): CY2120
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cre
• Species: Other
• Mutation: S102Y and S279Y- please see depositor comments

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pE_Cre_lambdaCosc.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor confirms the S102Y and S279Y in Cre does not affect plasmid function.

The pE_Cre_lambdaCos plasmid can be packaged in lambda phages using the CY2120 cosmid packaging strain [Cronan 2013]. Lambda infection provides a rapid and efficient way to deliver the pE_Cre_lambdaCos plasmid.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.