pHR-mIFP-NLS
(Plasmid
#173893)
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Purposefor mIFP expression in nucleus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173893 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHR
- Backbone size w/o insert (bp) 8514
- Total vector size (bp) 9534
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemIFP
- Promoter SSFV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer 1166 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR-mIFP-NLS was a gift from Ron Vale (Addgene plasmid # 173893 ; http://n2t.net/addgene:173893 ; RRID:Addgene_173893) -
For your References section:
High-content imaging-based pooled CRISPR screens in mammalian cells. Yan X, Stuurman N, Ribeiro SA, Tanenbaum ME, Horlbeck MA, Liem CR, Jost M, Weissman JS, Vale RD. J Cell Biol. 2021 Feb 1;220(2). pii: 211696. doi: 10.1083/jcb.202008158. 10.1083/jcb.202008158 PubMed 33465779
Map uploaded by the depositor.
