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pCMV-Spike-VLP
(Plasmid #173922)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 173922 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

* Login to view industry pricing.

Backbone

  • Vector backbone
    pUC57-Kan
  • Backbone size w/o insert (bp) 2539
  • Total vector size (bp) 8996
  • Modifications to backbone
    EcoRV on both ends allows linearization. LoxP allows rapid knock-in after re-circularization.
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SARS-2 Spike Protein
  • Species
    H. sapiens (human), Synthetic; SARS-COV-2
  • Insert Size (bp)
    3819
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV
  • Tag / Fusion Protein
    • no tags, native protein

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    SARS-Cov-2 E protein
  • Species
    H. sapiens (human), Synthetic; SARS-COV-2
  • Insert Size (bp)
    228
  • Entrez Gene
    E (a.k.a. GU280_gp04)
  • Promoter 22bp IRES

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    SARS-Cov-2 M Protein
  • Species
    H. sapiens (human), Synthetic; SARS-COV-2
  • Insert Size (bp)
    669
  • Entrez Gene
    M (a.k.a. GU280_gp05)
  • Promoter IE2 Promoter/Enhancer from Mouse MCMV

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Spike-VLP was a gift from Andreas Stuermer (Addgene plasmid # 173922 ; http://n2t.net/addgene:173922 ; RRID:Addgene_173922)