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pWZL hygro Flag HA TRAP220 wt
(Plasmid #17433)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17433 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pWZL Hygro
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRAP220
  • Alt name
    Med1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4700
  • GenBank ID
    NM_004774
  • Entrez Gene
    MED1 (a.k.a. CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2)
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZL hygro Flag HA TRAP220 wt was a gift from Kai Ge (Addgene plasmid # 17433 ; http://n2t.net/addgene:17433 ; RRID:Addgene_17433)
  • For your References section:

    Alternative mechanisms by which mediator subunit MED1/TRAP220 regulates peroxisome proliferator-activated receptor gamma-stimulated adipogenesis and target gene expression. Ge K, Cho YW, Guo H, Hong TB, Guermah M, Ito M, Yu H, Kalkum M, Roeder RG. Mol Cell Biol. 2008 Feb . 28(3):1081-91. 10.1128/MCB.00967-07 PubMed 18039840