pEGFP-Flag-stm2585
(Plasmid
#174397)
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PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174397 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-C1
- Total vector size (bp) 5286
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesarA
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Alt namestm2585
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Alt namesteE
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SpeciesSalmonella enterica ser. Typhimurium
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Insert Size (bp)573
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Tags
/ Fusion Proteins
- EGFP (N terminal on insert)
- Flag (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-Flag-stm2585 was a gift from Dennis Ko (Addgene plasmid # 174397 ; http://n2t.net/addgene:174397 ; RRID:Addgene_174397) -
For your References section:
The Salmonella Secreted Effector SarA/SteE Mimics Cytokine Receptor Signaling to Activate STAT3. Gibbs KD, Washington EJ, Jaslow SL, Bourgeois JS, Foster MW, Guo R, Brennan RG, Ko DC. Cell Host Microbe. 2020 Jan 8;27(1):129-139.e4. doi: 10.1016/j.chom.2019.11.012. Epub 2019 Dec 31. 10.1016/j.chom.2019.11.012 PubMed 31901521
