1
Plasmid 17458: pE4n
  • modified pENTR vector for N-terminal GST tag fusion with your gene of interest

  • None

  • GST

  • N terminal on backbone

  • Thrombin site

  • N terminal on backbone

  • pENTR1A
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression, Bacterial Expression, Worm Expression ; modified Gateway Entry vector

  • 2982

  • A short cloning site region and stop codon replaced ccdB gene in pENTR1A. Inserted GST tag and thrombin cleavage site

  • Gateway Cloning

  • pENTR-F (5'-CTACAAACTCTTCCTGTTAGTTAG-3') List of Sequencing Primers

  • pENTR-R (5'-ATGGCTCATAACACCCCTTG-3')

  • Kanamycin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • View map

  • Giovanna Benvenuto

  • UBMTA

Comments: 

Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal GST tagged Entry vector with a thrombin cleavage site for protein purification purposes. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.

The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).

GenBank accession number: EU334819

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A modified Gateway cloning strategy for overexpressing tagged proteins in plants. Dubin et al (Plant Methods. 2008 Jan 22. 4(1):3. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17458" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with
17464: pE4c
17457: pE3n
17463: pE3c
17456: pE2n
17441: pE1n