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Plasmid 17460: pE6n
  • modified pENTR vector for N-terminal EYFP tag fusion with your gene of interest

  • None

  • EYFP

  • N terminal on backbone

  • pENTR1A
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression ; modified Gateway Entry vector

  • 3030

  • A short cloning site region and stop codon replaced ccdB gene in pENTR1A. Inserted EYFP tag

  • Gateway Cloning

  • pENTR-F (5'-CTACAAACTCTTCCTGTTAGTTAG-3') List of Sequencing Primers

  • pENTR-R (5'-ATGGCTCATAACACCCCTTG-3')

  • Kanamycin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • View map

  • Giovanna Benvenuto

  • MTA

Comments: 

Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal EYFP tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.

The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).

GenBank accession number: EU344821

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A modified Gateway cloning strategy for overexpressing tagged proteins in plants. Dubin et al (Plant Methods. 2008 Jan 22. 4(1):3. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17460" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with
17466: pE6c
17459: pE5n
17465: pE5c
17457: pE3n
17462: pE2c