- Backbone size (bp) 3030
Modifications to backboneA short cloning site region and stop codon replaced ccdB gene in pENTR1A. Inserted EYFP tag
Vector typeMammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression ; modified Gateway Entry vector
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- EYFP (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer pENTR-F (5'-CTACAAACTCTTCCTGTTAGTTAG-3')
- 3′ sequencing primer pENTR-R (5'-ATGGCTCATAACACCCCTTG-3') (Common Sequencing Primers)
Terms and Licenses
Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal EYFP tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU344821
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pE6n was a gift from Giovanna Benvenuto (Addgene plasmid # 17460)
For your References section:A modified Gateway cloning strategy for overexpressing tagged proteins in plants. Dubin MJ, Bowler C, Benvenuto G. Plant Methods. 2008 Jan 22. 4(1):3. 10.1186/1746-4811-4-3 PubMed 18211686
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.