pCS2+WBP2NL-HA
(Plasmid
#174791)
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PurposeXenopus laevis WBP2NL with 3'HA tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174791 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4100
- Total vector size (bp) 5048
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameWBP2 N-terminal like
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Alt nameWBP2NL
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SpeciesX. laevis (frog)
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Insert Size (bp)942
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Mutationaa#165, Y to H; aa# 287, D to H
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GenBank IDBC022546.1
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Entrez Genewbp2nl.L (a.k.a. XELAEV_18023638mg, pawp, wbp2nl)
- Promoter SP6
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Cla1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+WBP2NL-HA was a gift from Sally Moody (Addgene plasmid # 174791 ; http://n2t.net/addgene:174791 ; RRID:Addgene_174791)