pDD2701
(Plasmid
#175078)
-
PurposeMammalian expression of Flag-tagged KSHV ORF45
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepcDNA3.1(+)
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameKSHV ORF45
-
Alt name46
-
SpeciesKaposi's sarcoma-associated herpesvirus 8
-
Entrez GeneORF45 (a.k.a. HHV8GK18_gp51)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byDr. Fanxiu Zhu (Florida State University), PMID: 19116250.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDD2701 was a gift from Dirk Dittmer (Addgene plasmid # 175078 ; http://n2t.net/addgene:175078 ; RRID:Addgene_175078) -
For your References section:
The ORF45 Protein of Kaposi Sarcoma-Associated Herpesvirus Is an Inhibitor of p53 Signaling during Viral Reactivation. Alzhanova D, Meyo JO, Juarez A, Dittmer DP. J Virol. 2021 Nov 9;95(23):e0145921. doi: 10.1128/JVI.01459-21. Epub 2021 Sep 15. 10.1128/JVI.01459-21 PubMed 34523970
