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PurposeMammalian expression vector for over-expression of anti-FLAG M2 light chain
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175358 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRK5
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameanti-FLAG M2 light chain
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Alt nameD54
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SpeciesM. musculus (mouse)
- Promoter CMV
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Tag
/ Fusion Protein
- N-terminal secretion signal peptide (MEAPAQLLFLLLLWLPDTTG) (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bysynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
anti-FLAG M2 light chain was a gift from Joost Snijder (Addgene plasmid # 175358 ; http://n2t.net/addgene:175358 ; RRID:Addgene_175358) -
For your References section:
Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies Using Multiple Proteases and a Dual Fragmentation Scheme. Peng W, Pronker MF, Snijder J. J Proteome Res. 2021 Jul 2;20(7):3559-3566. doi: 10.1021/acs.jproteome.1c00169. Epub 2021 Jun 14. 10.1021/acs.jproteome.1c00169 PubMed 34121409
