pDS1904
(Plasmid
#175596)
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Purpose(Empty Backbone) Integrative vector for Candida albicans with dominant SAT1 marker
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBluescript KS+
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is aimed to be introduced in the RPS1 locus of Candida albicans after StuI or NcoI digestion. It is derived from the plasmid CIp10 (Murad, A. M., Lee, P. R., Broadbent, I. D., Barelle, C. J. & Brown, A. J. CIp10, an efficient and convenient integrating vector for Candida albicans. Yeast 16, 325–327 (2000)), in which the URA3 marker was replaced by the selectable SAT1 dominant marker
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDS1904 was a gift from Dominique Sanglard (Addgene plasmid # 175596 ; http://n2t.net/addgene:175596 ; RRID:Addgene_175596) -
For your References section:
Red-Shifted Firefly Luciferase Optimized for Candida albicans In vivo Bioluminescence Imaging. Dorsaz S, Coste AT, Sanglard D. Front Microbiol. 2017 Aug 3;8:1478. doi: 10.3389/fmicb.2017.01478. eCollection 2017. 10.3389/fmicb.2017.01478 PubMed 28824601
Map uploaded by the depositor.
