tcTRP9sub3
(Plasmid #175794)

• Purpose: de novo thick circular tandem repeat protein trimer formed from 3-repeat monomers
• Depositing Lab: Barry Stoddard
• Publication: Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi: 10.1038/s42003-021-02766-y.

Backbone

• Vector backbone: pET15HE
• Backbone manufacturer: Stoddard Lab
• Backbone size w/o insert (bp): 5727
• Total vector size (bp): 6233
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tcTRP9sub3
• Species: Synthetic
• Insert Size (bp): 522
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GenScript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry