SH2-free
(Plasmid #175800)

• Purpose: Human Nck2 SH2 domain
• Depositing Lab: Barry Stoddard
• Publication: Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi: 10.1038/s42003-021-02766-y.

Backbone

• Vector backbone: pET15HE
• Backbone manufacturer: Stoddard Lab
• Backbone size w/o insert (bp): 5727
• Total vector size (bp): 6005
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human Nck2 SH2
• Species: H. sapiens (human)
• Insert Size (bp): 297
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • SH2-pET15HE.gb
• A portion of this plasmid was derived from a plasmid made by: Dr. Theresia Stradal of Helmholtz Centre for Infection Research

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gene was cloned from pGEX-6P1:huNck2-SH2 plasmid generously provided by Dr. Theresia Stradal of Helmholtz Centre for Infection Research