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pDest490-OPN-a
(Plasmid #17590)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17590 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDest-490
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OPN-a
  • Alt name
    OPN-FL
  • Alt name
    Osteopontin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    897
  • Mutation
    Base pairs 49–942
  • GenBank ID
    X13694
  • Entrez Gene
    SPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Mature human OPN cDNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest490-OPN-a was a gift from Xin Wang (Addgene plasmid # 17590 ; http://n2t.net/addgene:17590 ; RRID:Addgene_17590)
  • For your References section:

    An osteopontin fragment is essential for tumor cell invasion in hepatocellular carcinoma. Takafuji V, Forgues M, Unsworth E, Goldsmith P, Wang XW. Oncogene. 2007 Sep 27. 26(44):6361-71. 10.1038/sj.onc.1210463 PubMed 17452979