-3.6kb human IFN-gamma luc
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17599||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameIFN gamma promoter
Alt nameinterferon gamma
SpeciesH. sapiens (human)
Insert Size (bp)3670
Mutation-3.6 kb to +70 of promoter
Entrez GeneIFNG (a.k.a. IFG, IFI, IMD69)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Young lab stock #D3299.
SalI should give 8.5kb band
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:-3.6kb human IFN-gamma luc was a gift from Howard Young (Addgene plasmid # 17599 ; http://n2t.net/addgene:17599 ; RRID:Addgene_17599)
For your References section:Mucosa-specific targets for regulation of IFN-gamma expression: lamina propria T cells use different cis-elements than peripheral blood T cells to regulate transactivation of IFN-gamma expression. Gonsky R, Deem RL, Bream JH, Lee DH, Young HA, Targan SR. J Immunol. 2000 Feb 1. 164(3):1399-407. PubMed 10640755