pmr101A-cPr-mNeonGreen (ErmR)
(Plasmid
#177208)
-
PurposePlasmid with p15A origin for constitutive mNeonGreen expression in E. coli
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 177208 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepmr101A
- Total vector size (bp) 4222
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Erythromycin, 200 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namemNeonGreen
-
SpeciesBranchiostoma lanceolatum
-
Insert Size (bp)708
-
MutationYeast codon optimized
-
GenBank IDKC295282.1
- Promoter Synthetic constitutive promoter
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pmr101A forward mapping primer
- 3′ sequencing primer T7 Term (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byShaner NC, Lambert GG, Chammas A, Ni Y, Cranfill PJ, Baird MA, Sell BR, Allen JR, Day RN, Israelsson M, Davidson MW, Wang J. A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat Methods. 2013 May;10(5):407-9. doi: 10.1038/nmeth.2413. Epub 2013 Mar 24. PMID: 23524392; PMCID: PMC3811051.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Braatsch S, Helmark S, Kranz H, Koebmann B, Jensen PR. Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning. Biotechniques. 2008 Sep;45(3):335-7. doi: 10.2144/000112907. PMID: 18778259.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pmr101A-cPr-mNeonGreen (ErmR) was a gift from Alexey Merz (Addgene plasmid # 177208 ; http://n2t.net/addgene:177208 ; RRID:Addgene_177208)