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Addgene

pmr101A-cPr-mNeonGreen (ErmR)
(Plasmid #177208)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177208 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pmr101A
  • Total vector size (bp) 4222
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Erythromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mNeonGreen
  • Species
    Branchiostoma lanceolatum
  • Insert Size (bp)
    708
  • Mutation
    Yeast codon optimized
  • GenBank ID
    KC295282.1
  • Promoter Synthetic constitutive promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer pmr101A forward mapping primer
  • 3′ sequencing primer T7 Term
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Shaner NC, Lambert GG, Chammas A, Ni Y, Cranfill PJ, Baird MA, Sell BR, Allen JR, Day RN, Israelsson M, Davidson MW, Wang J. A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat Methods. 2013 May;10(5):407-9. doi: 10.1038/nmeth.2413. Epub 2013 Mar 24. PMID: 23524392; PMCID: PMC3811051.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Braatsch S, Helmark S, Kranz H, Koebmann B, Jensen PR. Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning. Biotechniques. 2008 Sep;45(3):335-7. doi: 10.2144/000112907. PMID: 18778259.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmr101A-cPr-mNeonGreen (ErmR) was a gift from Alexey Merz (Addgene plasmid # 177208 ; http://n2t.net/addgene:177208 ; RRID:Addgene_177208)