Addgene diagnostic digest
This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 1774 with SacII, EcoRI-HF, and HindIII-HF.
We expected the following banding patterns:
SacII single digest: 2 bands - 2.56 kb and 6.1 kb
EcoRI-HF single digest: 1 band at 8.7 kb
HindIII-HF single digest: 1 band at 8.7 kb
EcoRI-HF / HindIII-HF double digest: 2 bands - 3.7 kb and 4.9 kb