Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pBABE-neo-hTERT Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 1774 with SacII, EcoRI-HF, and HindIII-HF.

We expected the following banding patterns:

SacII single digest: 2 bands - 2.56 kb and 6.1 kb

EcoRI-HF single digest: 1 band at 8.7 kb

HindIII-HF single digest: 1 band at 8.7 kb

EcoRI-HF / HindIII-HF double digest: 2 bands - 3.7 kb and 4.9 kb