wg186Fluc
(Plasmid #177436)

• Purpose: In vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.
• Depositing Lab: Wendy Gilbert
• Publication: Rojas-Duran et al RNA. 2012 Dec;18(12):2299-305. doi: 10.1261/rna.035865.112. Epub 2012 Oct 25.

Backbone

• Vector backbone: wg186Fluc
• Backbone size w/o insert (bp): 2821
• Total vector size (bp): 4599
• Vector type: Luciferase ; For in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Firefly luciferase
• Alt name: Fluc
• Species: Synthetic; Photinus pyralis
• Insert Size (bp): 1778
• Promoter: T7
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nco1 (not destroyed)
• 3′ cloning site: Ecl136II (unknown if destroyed)
• 5′ sequencing primer: TAATACGACTCACTATAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Wendy Gilbert, Yale University
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry