pZS230
(Plasmid
#177645)
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PurposeBacterial expression of Patiria miniata Dishevelled 1-90
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177645 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX-6p
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDishevelled 1-90
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SpeciesPatiria miniata
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZS230 was a gift from Iain Cheeseman (Addgene plasmid # 177645 ; http://n2t.net/addgene:177645 ; RRID:Addgene_177645) -
For your References section:
Polarized Dishevelled dissolution and reassembly drives embryonic axis specification in sea star oocytes. Swartz SZ, Tan TH, Perillo M, Fakhri N, Wessel GM, Wikramanayake AH, Cheeseman IM. Curr Biol. 2021 Dec 20;31(24):5633-5641.e4. doi: 10.1016/j.cub.2021.10.022. Epub 2021 Nov 4. 10.1016/j.cub.2021.10.022 PubMed 34739818
