CJ26 (miR-1003 3'ssAG->GA)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17765||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDavid Sabatini Lab
Vector typeInsect Expression ; Drosophila metallothionein gene promoter drives expression
Growth in Bacteria
Gene/Insert namedme-miR-1003 minigene 3'ssAG->GA
SpeciesD. melanogaster (fly)
Insert Size (bp)390
Mutationsplice site mutation
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer MT forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Site-directed mutagenesis was used to make 3' splice site mutations with the indicated primers: CCTCTCACAT TTACATATTC ACGACGCCGT GAGCTGC and GCAGCTCACG GCGTCGTGAA TATGTAAATG TGAGAGG.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CJ26 (miR-1003 3'ssAG->GA) was a gift from David Bartel (Addgene plasmid # 17765 ; http://n2t.net/addgene:17765 ; RRID:Addgene_17765)
For your References section:Intronic microRNA precursors that bypass Drosha processing. Ruby JG, Jan CH, Bartel DP. Nature. 2007 Jul 5. 448(7149):83-6. 10.1038/nature05983 PubMed 17589500
Map uploaded by the depositor.