CJ31 (miR-1006 minigene)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17768||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCaSpeR4-tubulin promoter
Backbone manufacturerCarl Thummel Lab
- Backbone size w/o insert (bp) 10200
Vector typeDrosophila P-element vector
Growth in Bacteria
Gene/Insert namedme-miR-1006 minigene
SpeciesD. melanogaster (fly)
Insert Size (bp)770
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
Minigenes containing mir-1003 and mir-1006 and flanking exons were PCR amplified from genomic DNA. The sequence provided by the author is of the insert. pCaSpeR4 contains a ~2.4 kb tubulin promoter inserted between the EcoRI and KpnI sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CJ31 (miR-1006 minigene) was a gift from David Bartel (Addgene plasmid # 17768 ; http://n2t.net/addgene:17768 ; RRID:Addgene_17768)
For your References section:Intronic microRNA precursors that bypass Drosha processing. Ruby JG, Jan CH, Bartel DP. Nature. 2007 Jul 5. 448(7149):83-6. 10.1038/nature05983 PubMed 17589500