CJ45 (miR-1003 5' splice site mut)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17775||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerDavid Sabatini Lab
Vector typeInsect Expression ; Drosophila metallothionein gene promoter drives expression
Growth in Bacteria
Gene/Insert namedme-miR-1003 minigene 5' splice mut
SpeciesD. melanogaster (fly)
Insert Size (bp)390
Mutation5' splice site mut + compsensatory stem
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer MT forward (Common Sequencing Primers)
Terms and Licenses
Site-directed mutagenesis was used to make 5' splice site mutations with the indicated primers: GCTGCGCAGA ACGTGGGCAT CTGGATGTGG TTGGC and GCCAACCACA TCCAGATGCC CACGTTCTGC GCAGC; CCTCTCACAT TTACATGTTC ACAGGCGCCGT GAG and CTCACGGCGC CTGTGAACAT GTAAATGTGA GAGG.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CJ45 (miR-1003 5' splice site mut) was a gift from David Bartel (Addgene plasmid # 17775 ; http://n2t.net/addgene:17775 ; RRID:Addgene_17775)
For your References section:Intronic microRNA precursors that bypass Drosha processing. Ruby JG, Jan CH, Bartel DP. Nature. 2007 Jul 5. 448(7149):83-6. 10.1038/nature05983 PubMed 17589500
Map uploaded by the depositor.