CJ47 (U1 snRNA (U1;21d) 3G mutant)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17776||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2700
Growth in Bacteria
Gene/Insert nameU1 snRNA (U1;21d)
Alt nameU1 small nuclear RNA
SpeciesD. melanogaster (fly)
Entrez GenesnRNA:U1:21D (a.k.a. Dmel_CR31656, 21E U1, CR31656, Dmel\CR31656, K5, U1, U1 21D, U1 snRNA, U1-1, U1-21.1, U1-21D, U14, U1:21D, U1a, U1a-21D, U1a1(AT)[], U1snRNA, U1snRNA21D, dU1, snRNA-U1-21.1, snRNA1, snRNA:U1)
- Cloning method Restriction Enzyme
- 5′ sequencing primer m13 forward 20 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CJ47 (U1 snRNA (U1;21d) 3G mutant) was a gift from David Bartel (Addgene plasmid # 17776 ; http://n2t.net/addgene:17776 ; RRID:Addgene_17776)
For your References section:Intronic microRNA precursors that bypass Drosha processing. Ruby JG, Jan CH, Bartel DP. Nature. 2007 Jul 5. 448(7149):83-6. 10.1038/nature05983 PubMed 17589500