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CJ51 (cg1849 utr)
(Plasmid #17779)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pMT-puro with renilla luciferase
  • Backbone manufacturer
    pMT-puro from David Sabatini Lab
  • Vector type
    Insect Expression, Luciferase ; Drosophila metallothionein gene promoter drives expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    cg1849 utr fragment
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    450
  • GenBank ID
    CG1849
  • Tag / Fusion Protein
    • renilla luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer MT forward
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CJ51 (cg1849 utr) was a gift from David Bartel (Addgene plasmid # 17779)
  • For your References section:

    Intronic microRNA precursors that bypass Drosha processing. Ruby JG, Jan CH, Bartel DP. Nature. 2007 Jul 5. 448(7149):83-6. 10.1038/nature05983 PubMed 17589500