Addgene - CJ51 (cg1849 utr) Plasmid Data

Plasmid 17779: CJ51 (cg1849 utr)

Gene/insert name:
cg1849 utr fragment
Insert size:
450
Species:
D. melanogaster (fly)
GenBank ID:
CG1849
Fusion protein or tag:
renilla luciferase
Terminal:
N terminal on backbone
Vector backbone:
pMT-puro with renilla luciferase
(Search Vector Database)
Backbone manufacturer:
pMT-puro from David Sabatini Lab
Vector type:
Insect Expression, Luciferase ; Drosophila metallothionein gene promoter drives expression
Cloning site 5':
SpeI
Site destroyed during cloning:
Unknown
Cloning site 3':
NotI
Site destroyed during cloning:
Unknown
5' sequencing primer:
MT forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
David Bartel
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Intronic microRNA precursors that bypass Drosha processing. Ruby et al (Nature. 2007 Jul 5. 448(7149):83-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17779" in your Materials and Methods section.