pDEST-DHFR F[3]-C (LEU2)
(Plasmid
#177796)
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Purpose(Empty Backbone) Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177796 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDEST-DB
- Backbone size (bp) 10151
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Modifications to backboneRemoved DB used for Y2H, and inserted DHFR F[3] fragment. Inserted I-CeuI, I-SceI, and PI-PspI homing endonuclease sites on backbone.
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Vector typeYeast Expression
- Promoter ADH1
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Selectable markersLEU2
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Tag
/ Fusion Protein
- DHFR F[3] (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsThe chloramphenicol resistance gene in the Gateway cassette is broken.
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.07.27.453987v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST-DHFR F[3]-C (LEU2) was a gift from Christian Landry (Addgene plasmid # 177796 ; http://n2t.net/addgene:177796 ; RRID:Addgene_177796) -
For your References section:
Barcode fusion genetics-protein-fragment complementation assay (BFG-PCA): tools and resources that expand the potential for binary protein interaction discovery. Evans-Yamamoto D, Rouleau FD, Nanda P, Makanae K, Liu Y, Despres PC, Matsuo H, Seki M, Dube AK, Ascencio D, Yachie N, Landry CR. Nucleic Acids Res. 2022 Feb 7. pii: 6523796. doi: 10.1093/nar/gkac045. 10.1093/nar/gkac045 PubMed 35137167
