pSUMO His6 SUMO PYL1 (36-211)
(Plasmid
#177843)
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PurposeBacterial Expression of PYL1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177843 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSUMO
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Backbone manufacturerLife Sensors
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePYR1-like 1
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SpeciesA. thaliana (mustard weed)
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Entrez GenePYL1 (a.k.a. AT5G46790, MZA15.21, MZA15_21, PYR1-like 1, RCAR12, regulatory components of ABA receptor 12)
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Tag
/ Fusion Protein
- 6xHis-SUMO (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 3′ sequencing primer T7term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSUMO His6 SUMO PYL1 (36-211) was a gift from Karsten Melcher (Addgene plasmid # 177843 ; http://n2t.net/addgene:177843 ; RRID:Addgene_177843) -
For your References section:
A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Melcher K, Ng LM, Zhou XE, Soon FF, Xu Y, Suino-Powell KM, Park SY, Weiner JJ, Fujii H, Chinnusamy V, Kovach A, Li J, Wang Y, Li J, Peterson FC, Jensen DR, Yong EL, Volkman BF, Cutler SR, Zhu JK, Xu HE. Nature. 2009 Dec 3;462(7273):602-8. doi: 10.1038/nature08613. 10.1038/nature08613 PubMed 19898420
