Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

CJ64 (miR-1003 5' splice site mut)
(Plasmid #17785)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17785 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCaSpeR4-tubulin promoter
  • Backbone size w/o insert (bp) 10200
  • Vector type
    Drosophila P-element vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dme-miR-1003 minigene 5' splice mut
  • Alt name
    miR-1003
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    390
  • Mutation
    5' splice site mut + compsensatory stem

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Site-directed mutagenesis was used to make 5' splice site mutations with the indicated primers: GCTGCGCAGA ACGTGGGCAT CTGGATGTGG TTGGC and GCCAACCACA TCCAGATGCC CACGTTCTGC GCAGC; CCTCTCACAT TTACATGTTC ACAGGCGCCG TGAG and CTCACGGCGC CTGTGAACAT GTAAATGTGA GAGG. pCaSpeR4 contains a ~2.4 kb tubulin promoter inserted between the EcoRI and KpnI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CJ64 (miR-1003 5' splice site mut) was a gift from David Bartel (Addgene plasmid # 17785 ; http://n2t.net/addgene:17785 ; RRID:Addgene_17785)
  • For your References section:

    Intronic microRNA precursors that bypass Drosha processing. Ruby JG, Jan CH, Bartel DP. Nature. 2007 Jul 5. 448(7149):83-6. 10.1038/nature05983 PubMed 17589500