pSumo-His6- Sumo-OsTPL(1-209)
(Plasmid
#177855)
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PurposeBacterial Expression of TPL
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177855 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSUMO
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Backbone manufacturerLife Sensors
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTPL
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SpeciesOryza sativa (rice)
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Tag
/ Fusion Protein
- 6xHis-SUMO (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSumo-His6- Sumo-OsTPL(1-209) was a gift from Karsten Melcher (Addgene plasmid # 177855 ; http://n2t.net/addgene:177855 ; RRID:Addgene_177855) -
For your References section:
Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors. Ke J, Ma H, Gu X, Thelen A, Brunzelle JS, Li J, Xu HE, Melcher K. Sci Adv. 2015 Jul 24;1(6):e1500107. doi: 10.1126/sciadv.1500107. eCollection 2015 Jul. 10.1126/sciadv.1500107 PubMed 26601214
