PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||177938||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Gibson Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://www.biorxiv.org/content/10.1101/2021.08.05.455082v1 for bioRxv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CoV2-M-IRES-E was a gift from Jennifer Doudna (Addgene plasmid # 177938 ; http://n2t.net/addgene:177938 ; RRID:Addgene_177938)
For your References section:Rapid assessment of SARS-CoV-2 evolved variants using virus-like particles. Syed AM, Taha TY, Tabata T, Chen IP, Ciling A, Khalid MM, Sreekumar B, Chen PY, Hayashi JM, Soczek KM, Ott M, Doudna JA. Science. 2021 Nov 4:eabl6184. doi: 10.1126/science.abl6184. 10.1126/science.abl6184 PubMed 34735219